Vyskočil (feminine: Vyskočilová) is a Czech surname. Alternative spellings include Wiskocil, Wiskoczil, and Wyskocil.
Notable people with the surname include:
František Vyskočil (born 1941), Czech neuroscientist
Ivan Vyskočil (born 1946), Czech actor
Martin Vyskočil (born 1982), Czech footballer
Mary Kay Vyskocil (born 1958), United States District Judge
Pavel Vyskočil (1882–1970), Czech opera singer
Rudolf Wiskoczil (1870–1925), Austrian architect
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