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501 Queen (301 Queen during overnight periods) is an east–west Toronto streetcar route in Ontario, Canada, operated by the Toronto Transit Commission (TTC). It stretches from Neville Park Loop (just west of Victoria Park Avenue) in the east, running along Queen Street and in a reserved right-of-way within the median of the Queensway to Humber Loop in the west. In the late evenings, the 501 Queen route is extended west from Humber Loop, running on Lake Shore Boulevard to Long Branch Loop (just west of Browns Line, adjacent to Long Branch GO Station), replacing route 507 Long Branch. This route operates as part of the TTC's Blue Night Network service, operating in the early morning hours as the 301 Queen.
In 2022, with almost 12.8 million annual boardings, 501 Queen was the second busiest streetcar line in Toronto, after 504 King. (In 2019, prior to the drop in ridership due to the COVID-19 pandemic, there were 22.0 million annual boardings.)

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